Metabolomics and Natural-Products Strategies to Study Chemical Ecology in Nematodes

bodnar, george. h.;

This review provides an overview of two complementary approaches to identify biologically active compounds for studies in chemical ecology. The first is activity-guided fractionation and the second is metabolomics, particularly focusing on a new liquid chromatography–mass spectrometry-based method called isotopic ratio outlier analysis. To illustrate examples using these approaches, we review recent experiments using Caenorhabditis elegans and related freeliving nematodes.