Insights on the Evolution of My coparasitism from the Genome of Clonostachys rosea

alvina nisya rahmawati;

Clonostachys rosea is a mycoparasitic fungus that can control several important plant diseases. Here, we report on the genome sequencing ofC. rosea and acomparative genome analysis, in order to resolve the phylogenetic placement of C.rosea and to studythe evolution of mycoparasitism as a fungal lifestyle. The genome of C. rosea is estimated to 58.3Mb, and contains 14,268 predicted genes. A phylogenomicanalysis shows that C. rosea clusters assister taxontoplant pathogenic Fusarium species, with mycoparasitic/ saprotrophic Trichoderma species in an ancestral position. A comparative analysis of gene family evolution reveals several distinct differences between the included mycoparasites. Clonostachys rosea contains significantly more ATP-binding cassette (ABC) transporters, polyketide synthases, cytochrome P450 monooxygenases, pectin lyases, glucose-methanol-choline oxidoreductases, and lytic polysaccharide monooxygenases compared with other fungi in the Hypocreales. Interestingly, the increase of ABC transporter gene number in C.rosea is associated with phylogenetic subgroups B (multi drug resistance proteins) and G(pleio tropic drug resistance transporters), whereas an increase in subgroup C (multidrug resistance-associated proteins) is evident in Trichoderma virens. In contrast with mycoparasitic Trichoderma species, C. rosea contains very few chitinases. Expression of six group B and group G ABC transporter genes was induced in C. rosea during exposure to the Fusarium mycotoxin zearalenone, the fungicide Boscalid or metabolites from the biocontrol bacterium Pseudomonas chlororaphis. The data suggest that tolerance toward secondary metabolites is a prominent feature in the biology of C. rosea